Association of COL2A1rs2276454, rs1793953, COL9A1rs1135056, COL11A1rs1676486 structure-forming collagens of the nucleus pulposus with degeneration of intervertebral discs L5-L4, L5-S1

Aim. The purpose of the work was to determine relationship between degenerative changes L5-S1 and L4-L5 intervertebral discs with collagen mononucleotide variants COL2A1rs2276454, rs1793953, COL9A1rs1135056, COL11A1rs1676486 among ethnic Ukrainians. Materials methods. following subjects were investigated: 90 persons case group degeneration disc L5-S1; 50 L4-L5; 66 people control group. object study venous blood patients lesions healthy donors. Venous obtained as a result venipuncture. Typing COL2A1rs1793953, performed using Tag Man Universal PCR Master Mix kit (Applied Biosystems, USA) SNP Genotyping Assays for determination polymorphisms USA). research carried out by specialists in field molecular biology biochemistry Department Neurobiochemistry SI “Romodanov Neurosurgery Institute, National Academy Medical Sciences Ukraine”, CFX96 device (Bio-Rrad, Certificate measuring capabilities No. PT-322/21 from 07/28/2021 08/27/2023. Results. COL2A1rs2276454 may have protective value development men (OR (95 % CI): 0.27 (0.10–0.80), χ2 = 6.02, P 0.015). Genotype C/T (COL9A1rs1135056) is 3.25 times more common male comparison female (1.20–8.84), 5.50, 0.02). presence genotype G/A (COL11A1rs1676486) general observed 5.46 often (1.60–18.47), 8.29, 0.004); registered 4.17 compared women (1.070–16.82), 4.17, 0.04). Comparison odds observing G/G, G/A, AA showed statistically significant 4.06 predominance (1.23–13.38), most probable model heredity dominant OR 2.08 (1.03–4.21), 4.26, 0.04. Associations structure-forming collagens gelatinous nucleus L5-L4 not detected. Conclusions. be Р COL2A1rs1793953 associated L4-L5, L5-S1. С/T COL9A3rs1135056 males 0.02) females. 0.004) (1.07–16.82), type inheritance dominant.